Rabbit anti-PSME4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-880A-T maps to a region between residue 1 and 50 of human Proteasome (Prosome, Macropain) Activator subunit 4 using the numbering given in entry NP_055429.2 (GeneID 23198). Antibody was affinity purified using an epitope specific to PSME4 immobilized on solid support.
The epitope recognized by A303-880A maps to a region between residue 1 and 50 of human Proteasome (Prosome, Macropain) Activator subunit 4 using the numbering given in entry NP_055429.2 (GeneID 23198).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
proteasome (prosome, macropain) activator subunit 4 (PSME4) is subunit of the proteasome. PSME4 has been found to activate proteasomal cleavage of peptides in an energy-independent manner and may be involved in spermatogenesis. PSME4 may also be involved in DNA repair [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q14997].
Alternate Names
PA200; proteasome (prosome, macropain) activator subunit 4; proteasome activator 200 kDa; proteasome activator complex subunit 4; proteasome activator PA200
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.