Rabbit anti-PSMF1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-859A maps to a region between residue 150 and 200 of human Proteasome (Prosome, Macropain) Activator Subunit 1 (PI31) using the numbering given in entry NP_848693.2 (GeneID 9491).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PSMF1 immobilized on solid support.
The epitope recognized by A303-859A-T maps to a region between residue 150 and 200 of human Proteasome (Prosome, Macropain) Activator Subunit 1 (PI31) using the numbering given in entry NP_848693.2 (GeneID 9491).
Additional Product Information
PSMF1 [proteasome (prosome, macropain) inhibitor subunit 1 (PI31)] plays an important role in control of proteasome function. It inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. PSMF1 also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92530].
Alternate Names
hPI31; PI31; proteasome (prosome, macropain) inhibitor subunit 1 (PI31); proteasome inhibitor PI31 subunit
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.