Rabbit anti-PTP1B Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-298A-T maps to a region between residue 385 and 435 of human protein tyrosine phosphatase 1B using the numbering given in entry NP_002818.1 (GeneID 5770).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PTP1B immobilized on solid support.
The epitope recognized by A302-298A maps to a region between residue 385 and 435 of human protein tyrosine phosphatase 1B using the numbering given in entry NP_002818.1 (GeneID 5770).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Protein-tyrosine phosphatases 1B (PTP1B) is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTP1B has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
Alternate Names
protein tyrosine phosphatase, placental; protein-tyrosine phosphatase 1B; PTP1B; PTP-1B; tyrosine-protein phosphatase non-receptor type 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.