NEW!

Rabbit anti-Pur-alpha Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
Pur-alpha
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 125 and 175
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Request Formulation Change
Production Procedures
Antibody was affinity purified using an epitope specific to Pur-alpha immobilized on solid support.

The epitope recognized by A303-544A-T maps to a region between residue 100 and 150 of human Purine-Rich Element Binding Protein Alpha using the numbering given in entry NP_005850.1 (GeneID 5813).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to Pur-alpha immobilized on solid support.

The epitope recognized by A303-544A maps to a region between residue 100 and 150 of human Purine-Rich Element Binding Protein Alpha using the numbering given in entry NP_005850.1 (GeneID 5813).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Transcriptional activator protein pur-alpha (purine-rich single stranded DNA-binding protein alpha) is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. Pur-alpha may play a role in the initiation of DNA replication and in recombination [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q00577].

5813
PURA
purine rich element binding protein A
Q00577
Transcriptional activator protein Pur-alpha

Alternate Names

MRD31; PUR1; PURALPHA; PUR-ALPHA; purine-rich single-stranded DNA-binding protein alpha; transcriptional activator protein Pur-alpha

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.