Rabbit anti-Pur-alpha Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-544A-T maps to a region between residue 100 and 150 of human Purine-Rich Element Binding Protein Alpha using the numbering given in entry NP_005850.1 (GeneID 5813).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Pur-alpha immobilized on solid support.
The epitope recognized by A303-544A maps to a region between residue 100 and 150 of human Purine-Rich Element Binding Protein Alpha using the numbering given in entry NP_005850.1 (GeneID 5813).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Transcriptional activator protein pur-alpha (purine-rich single stranded DNA-binding protein alpha) is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. Pur-alpha may play a role in the initiation of DNA replication and in recombination [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q00577].
Alternate Names
MRD31; PUR1; PURALPHA; PUR-ALPHA; purine-rich single-stranded DNA-binding protein alpha; transcriptional activator protein Pur-alpha
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.