Rabbit anti-PVRL1/NECTIN1 Recombinant Monoclonal Antibody [BLR274L]
Product Details
Specifications
Immunogen was a recombinant protein representing the extracellular domain of human PVRL1/NECTIN1 (residues 31-331, Gene ID 5818).
Additional Product Information
PVRL1/NECTIN1 (aka CD111) is an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily. It acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2) and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells [taken from NCBI Entrez Gene (Gene ID: 5818)].
Alternate Names
CD antigen CD111;CD111;CLPED1;ectodermal dysplasia 4 (Margarita Island type);ED4;herpes simplex virus type 1 sensitivity;herpes virus entry mediator C;Herpesvirus entry mediator C;herpesvirus Ig-like receptor;HIgR;HV1S;HVEC;nectin 1;Nectin cell adhesion molecule 1;nectin-1;OFC7;poliovirus receptor-like 1;poliovirus receptor-related 1 (herpesvirus entry mediator C);poliovirus receptor-related protein 1;PRR;PRR1;PVRL1;PVRR;PVRR1;SK-12
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.