Rabbit anti-Pygo1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-648A-T maps to a region between residue 300 and 350 of human pygopus homolog 1 using the numbering given in entry NP_056432.1 (GeneID 26108).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Pygo1 immobilized on solid support.
The epitope recognized by A302-648A maps to a region between residue 300 and 350 of human pygopus homolog 1 using the numbering given in entry NP_056432.1 (GeneID 26108 ).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Pygo1 (pygopus homolog 1) is the mammalian homolog of the drosophila pygopus protein. Pygo1 is involved in signal transduction through the Wnt pathway.
Alternate Names
pygopus homolog 1; pygopus-like protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.