Rabbit anti-RAB1A Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-327A maps to a region between residue 1 to 50 of human Ras-related protein Rab-1A using the numbering given in entry P62820.3 (GeneID 5861).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RAB1A immobilized on solid support.
The epitope recognized by A305-327A-T maps to a region between residue 1 to 50 of human Ras-related protein Rab-1A using the numbering given in entry P62820.3 (GeneID 5861).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
RAB1A is a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus [taken from NCBI Entrez Gene (Gene ID: 5861)].
Alternate Names
GTP binding protein Rab1a; Rab GTPase YPT1 homolog; RAB1; RAB1, member RAS oncogene family; ras-related protein Rab-1A; YPT1; YPT1-related protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.