Rabbit anti-Rab3-GAP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-113A maps to a region between residue 350 and 400 of human RAB3 GTPase activating protein subunit 1 using the numbering given in entry NP_036365.1 (GeneID 22930).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Rab3-GAP1 immobilized on solid support.
The epitope recognized by A302-113A-T maps to a region between residue 350 and 400 of human RAB3 GTPase activating protein subunit 1 using the numbering given in entry NP_036365.1 (GeneID 22930).
Additional Product Information
Rab3-GAP1 is the catalytic subunit of Rab3-GAP. Rab3-GAP is a key regulator of calcium-mediated hormone and neurotransmitter exocytosis. Defects in Rab3-GAP1 are the cause of Warburg micro syndrome 1 (WARBM1) a disorder characterized by severe developmental abnormalities of the eye and central nervous system.
Alternate Names
P130; RAB3 GTPase activating protein subunit 1 (catalytic); RAB3 GTPase-activating protein 130 kDa subunit; rab3 GTPase-activating protein catalytic subunit; RAB3GAP; Rab3-GAP; rab3-GAP p130; RAB3GAP130; WARBM1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.