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Rabbit anti-RAI1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
RAI1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 25 and 75
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to RAI1 immobilized on solid support.

The epitope recognized by A302-317A maps to a region between residue 25 and 75 of human retinoic acid induced 1 using the numbering given in entry NP_109590.3 (GeneID 10743).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to RAI1 immobilized on solid support.

The epitope recognized by A302-317A-T maps to a region between residue 25 and 75 of human retinoic acid induced 1 using the numbering given in entry NP_109590.3 (GeneID 10743).
Country of Origin
USA

Additional Product Information

Defects in Retinoic acid-induced protein 1 (RAI1) are a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. SMS is characterized by congenital mental retardation associated with development and growth delays. The RAI1 has also been associated with schizophrenia [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q7Z5J4].

10743
RAI1
retinoic acid induced 1
Q7Z5J4
Retinoic acid-induced protein 1

Alternate Names

retinoic acid-induced protein 1; SMCR; Smith-Magenis syndrome chromosome region; SMS

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.