Rabbit anti-RanBP2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-797A maps to a region between residue 2600 and 2650 of human RAN binding protein 2 using the numbering given in entry NP_006258.3 (GeneID 5903).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RanBP2 immobilized on solid support.
The epitope recognized by A301-797A-T maps to a region between residue 2600 and 2650 of human RAN binding protein 2 using the numbering given in entry NP_006258.3 (GeneID 5903).
Additional Product Information
Ran binding protein 2 (RanBP2) is a large multi-modular nucleoporin protein (also called NUP358) that bears several structural domains including a cyclophilin-type rotamase domain, four RanBD1 domains, eight RanBP2-type zinc fingers, and one TPR repeat. This variety of structural domains imparts pleiotropic qualities to RanBP2. RanBP2 has been shown to function as an E3 SUMO-protein ligase and is also part of the nuclear export pathway.
Alternate Names
358 kDa nucleoporin; acute necrotizing encephalopathy 1 (autosomal dominant); ADANE; ANE1; E3 SUMO-protein ligase RanBP2; E3 SUMO-protein transferase RanBP2; IIAE3; nuclear pore complex protein Nup358; nucleoporin 358; nucleoporin Nup358; NUP358; P270; ran-binding protein 2; RanBP2; transformation-related protein 2; TRP1; TRP2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.