Rabbit anti-RC3H2/MNAB Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-150A-T maps to a region between residue 1100 to 1150 of human RING finger and CCCH-type zinc finger domain-containing protein 2 using the numbering given in entry Q9HBD1.2 (GeneID 54542).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RC3H2/MNAB immobilized on solid support.
The epitope recognized by A305-150A maps to a region between residue 1100 to 1150 of human RING finger and CCCH-type zinc finger domain-containing protein 2 using the numbering given in entry Q9HBD1.2 (GeneID 54542).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Roquin-2 is encoded by the gene RC3H2, also known as MNAB. Roquin-2 is a post-transcriptional repressor of mRNAs containing a conserved stem loop motif called the constitutive decay element (CDE). Binding to the CDE promotes mRNA deadenylation and degradation [taken from UniProtKB (Entry: Q9HBD1)].
Alternate Names
membrane associated DNA binding protein; membrane-associated nucleic acid-binding protein; MNAB; ring finger and CCCH-type zinc finger domain-containing protein 2; ring finger and CCCH-type zinc finger domains 2; RING finger protein 164; RING-type E3 ubiquitin transferase Roquin-2; RNF164; roquin-2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.