Rabbit anti-RCC1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-604A-T maps to a region between residue 200 and 250 of human regulator of chromosome condensation 1 using the numbering given in entry NP_001260.1 (GeneID 1104).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RCC1 immobilized on solid support.
The epitope recognized by A302-604A maps to a region between residue 200 and 250 of human regulator of chromosome condensation 1 using the numbering given in entry NP_001260.1 (GeneID 1104 ).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
RCC1 (regulator of chromosome condensation 1) is a chromatin associated guanine-nucleotide exchange factor (GEF) for the small nuclear G-protein, Ran. RCC1 is important to the nuclear pore transport process as well as chromosome condensation during S-phase of mitosis. The RCC1/Ran complex may also function to communicate the status of chromosomal replication to the events of cell cycle progression.
Alternate Names
cell cycle regulatory protein; CHC1; Chromosome condensation protein 1; guanine nucleotide-releasing protein; RCC1-I; regulator of chromosome condensation; SNHG3-RCC1; SNHG3-RCC1 readthrough
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.