Rabbit anti-RCL Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-681A-T maps to a region between residue 124 to 174 of human c-Myc-Responsive Protein Rcl using the numbering given in entry NP_006434.1 (GeneID 10591). Antibody was affinity purified using an epitope specific to RCL immobilized on solid support.
The epitope recognized by A304-681A maps to a region between residue 124 to 174 of human c-Myc-Responsive Protein Rcl using the numbering given in entry NP_006434.1 (GeneID 10591).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
RCL catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43598].
Alternate Names
2'-deoxynucleoside 5'-phosphate N-hydrolase 1; C6orf108; c-Myc-responsive protein RCL; deoxyribonucleoside 5'-monophosphate N-glycosidase; dJ330M21.3; putative c-Myc-responsive; RCL
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.