Rabbit anti-RCOR3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-273A maps to a region between residue 445 and 495 of human REST corepressor 3 using the numbering given in entry NP_060724.1 (GeneID 55758).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RCOR3 immobilized on solid support.
The epitope recognized by A301-273A-T maps to a region between residue 445 and 495 of human REST corepressor 3 using the numbering given in entry NP_060724.1 (GeneID 55758).
Additional Product Information
RCOR3 is a member of the coREST family that functions as a co-repressor for REST (RE1-silencing transcription factor) to regulate neuronal gene expression and neuronal stem cell fate. The coREST family includes RCOR1, also known as coREST, RCOR2, and RCOR3. REST complexes regulate gene expression by modifying chromatin to create a repressive epigenetic environment.
Alternate Names
REST corepressor 3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.