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Rabbit anti-RecQ1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
RecQ1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 599 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to RecQ1 immobilized on solid support.

The epitope recognized by A300-450A maps to a region between residue 600 and the C-terminus (residue 649) of human RecQ protein-like (DNA helicase Q1-like) 1 using the numbering given in Swiss-Prot entry P46063 (GeneID 5965).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to RecQ1 immobilized on solid support.

The epitope recognized by A300-450A-T maps to a region between residue 600 and the C-terminus (residue 649) of human RecQ protein-like (DNA helicase Q1-like) 1 using the numbering given in Swiss-Prot entry P46063 (GeneID 5965).
Country of Origin
USA

Additional Product Information

RecQ1 is a member of the RecQ family of DNA helicases that functions in DNA repair, recombination, and replication and is important to the maintenance of genomic stability.

5965
RECQL
RecQ like helicase
P46063
ATP-dependent DNA helicase Q1

Alternate Names

ATP-dependent DNA helicase Q1; DNA helicase, RecQ-like type 1; DNA-dependent ATPase Q1; RecQ helicase-like; RecQ protein-like (DNA helicase Q1-like); recQ protein-like 1; RecQ1; RECQL1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.