Rabbit anti-REEP3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-662A maps to a region between residue 205 to 255 of human Receptor expression-enhancing protein 3 using the numbering given in entry Q6NUK4.1 (GeneID 221035).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to REEP3 immobilized on solid support.
The epitope recognized by A305-662A-T maps to a region between residue 205 to 255 of human Receptor expression-enhancing protein 3 using the numbering given in entry Q6NUK4.1 (GeneID 221035).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Receptor expression-enhancing protein 3 (REEP3) is a microtubule-binding protein required to ensure proper cell division and nuclear envelope reassembly by sequestering the endoplasmic reticulum away from chromosomes during mitosis. REEP3 probably acts by clearing the endoplasmic reticulum membrane from metaphase chromosomes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6NUK4].
Alternate Names
C10orf74; receptor expression-enhancing protein 3; Yip2b
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.