Rabbit anti-RelA Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-824A maps to a region between residue 501 and 551 of human reticuloendotheliosis viral oncogene homolog A using the numbering given in entry NP_068810.2 (GeneID 5970).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RelA immobilized on solid support.
The epitope recognized by A301-824A-T maps to a region between residue 501 and 551 of human reticuloendotheliosis viral oncogene homolog A using the numbering given in entry NP_068810.2 (GeneID 5970).
Additional Product Information
RelA is one of five monomeric subunits that are part of the Rel family of proteins that dimerize to form the NF-kappa B (nuclear factor that binds the kappa immunoglobulin light chain gene enhancer) transcription factor. Members of the Rel family of proteins include NF-kappa B1/p50, NF-kappa B2/p52, RelA/p65, c-Rel, and RelB. Each Rel member bears a characteristic 300 amino acid N-terminal Rel homology domain (RHD) that functions to mediate DNA binding, dimerization, nuclear localization, and inhibitor association.
Alternate Names
NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NFKB3; nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.