Rabbit anti-RGL2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-592A maps to a region between residue 727 to 777 of human Ral guanine nucleotide dissociation stimulator-like 2 using the numbering given in entry O15211.1 (GeneID 5863).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RGL2 immobilized on solid support.
The epitope recognized by A305-592A-T maps to a region between residue 727 to 777 of human Ral guanine nucleotide dissociation stimulator-like 2 using the numbering given in entry O15211.1 (GeneID 5863).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Ral guanine nucleotide dissociation stimulator-like 2 (RGL2) is a probable guanine nucleotide exchange factor It is a putative effector of Ras and/or Rap. RGL2 associates with the GTP-bound form of Rap 1A and H-Ras in vitro (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O15211].
Alternate Names
GDS-related protein; HKE1.5; KE1.5; RAB2L; ral guanine nucleotide dissociation stimulator-like 2; ralGDS-like 2; ralGDS-like factor; ras-associated protein RAB2L
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.