Rabbit anti-RGS14 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Regulator of G-protein signaling 14 (RGS14) acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). RGS14 may act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43566].
Alternate Names
regulator of G-protein signaling 14; regulator of G-protein signalling 14; RGS14
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.