Rabbit anti-RNF25 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-844A maps to a region between residue 409 and 459 of human Ring Finger Protein 25 using the numbering given in entry NP_071898.2 (GeneID 64320).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RNF25 immobilized on solid support.
The epitope recognized by A303-844A-T maps to a region between residue 409 and 459 of human Ring Finger Protein 25 using the numbering given in entry NP_071898.2 (GeneID 64320).
Additional Product Information
RNF25 (RING finger protein 25) is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (by similarity). RNF25 stimulates transcription mediated by NF-kappa-B [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96BH1].
Alternate Names
AO7; E3 ubiquitin-protein ligase RNF25; RING finger protein 25; ring finger protein AO7; RING-type E3 ubiquitin transferase RNF25
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.