Rabbit anti-RNF5 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
RNF5 (ring finger protein 5) is a membrane-bound E3 ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions [taken from NCBI Entrez Gene (Gene ID: 6048)].
Alternate Names
E3 ubiquitin-protein ligase RNF5; HsRma1; protein G16; ram1 homolog; RING finger protein 5; ring finger protein 5, E3 ubiquitin protein ligase; RING5; RING-type E3 ubiquitin transferase RNF5; RMA1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.