Rabbit anti-RPC3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-063A-T maps to a region between residue 200 and 250 of human Polymerase (RNA) III (DNA directed) Polypeptide C (62kD) using the numbering given in entry NP_006459.3 (GeneID 10623).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RPC3 immobilized on solid support.
The epitope recognized by A303-063A maps to a region between residue 200 and 250 of human Polymerase (RNA) III (DNA directed) Polypeptide C (62kD) using the numbering given in entry NP_006459.3 (GeneID 10623).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
RNA polymerase III subunit C (RPC3) encodes one of 17 subunits of the RNA polymerase III (PolIII) which synthesizes small RNAs, such as 5S rRNA and tRNA. The protein is encoded by the POLR3D gene.
Alternate Names
DNA-directed III 62 kDa polypeptide; DNA-directed RNA polymerase III subunit C; DNA-directed RNA polymerase III subunit RPC3; polymerase (RNA) III (DNA directed) polypeptide C (62kD); polymerase (RNA) III subunit C; RNA polymerase III 62 kDa subunit; RNA polymerase III subunit C3; RPC3; RPC62
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.