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Rabbit anti-RPN1/Ribophorin I Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
RPN1/Ribophorin I
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to RPN1/Ribophorin I immobilized on solid support.

The epitope recognized by A305-026A maps to a region between residue 1 to 50 of human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 using the numbering given in entry P04843.1 (GeneID 6184).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to RPN1/Ribophorin I immobilized on solid support.

The epitope recognized by A305-026A-T maps to a region between residue 1 to 50 of human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 using the numbering given in entry P04843.1 (GeneID 6184).
Country of Origin
USA

Additional Product Information

Ribophorin I is an essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains [taken from NCBI Entrez Gene (Gene ID: 6184)].

6184
RPN1
ribophorin I
P04843
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

Alternate Names

dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); OST1; RBPH1; Ribophorin I; ribophorin-1; RPN-I

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).