Rabbit anti-RPRD1B Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-782A maps to a region between residue 276 and 326 of human Regulation of Nuclear Pre-mRNA Domain Containing 1B using the numbering given in entry NP_067038.1 (GeneID 58490).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RPRD1B immobilized on solid support.
The epitope recognized by A303-782A-T maps to a region between residue 276 and 326 of human Regulation of Nuclear Pre-mRNA Domain Containing 1B using the numbering given in entry NP_067038.1 (GeneID 58490).
Additional Product Information
RPRD1B (regulation of nuclear pre-mRNA domain containing 1B) Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. RPRD1B is a transcriptional regulator that promotes cell proliferation and enhances the transcription of a number of cell cycle-related genes including CCND1, CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NQG5].
Alternate Names
C20orf77; Cell cycle-related and expression-elevated protein in tumor; cell-cycle related and expression-elevated protein in tumor; CREPT; dJ1057B20.2; K-H; Ku70-binding protein 5-Hera; Kub5-Hera; NET60; regulation of nuclear pre-mRNA domain-containing protein 1B
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.