Rabbit anti-RPSA Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-299A maps to a region between residue 245 to 295 of human 40S ribosomal protein SA using the numbering given in entry P08865.4 (GeneID 3921).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RPSA immobilized on solid support.
The epitope recognized by A305-299A-T maps to a region between residue 245 to 295 of human 40S ribosomal protein SA using the numbering given in entry P08865.4 (GeneID 3921).
Additional Product Information
RPSA is required for the assembly and/or stability of the 40S ribosomal subunit. RPSA is required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. RPSA also functions as a cell surface receptor for laminin and plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. RPSA may play a role in cell fate determination and tissue morphogenesis. It acts as a PPP1R16B-dependent substrate of PPP1CA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08865].
Alternate Names
37 kDa laminin receptor; 37 kDa laminin receptor precursor; 37/67 kDa laminin receptor; 37LRP; 40S ribosomal protein SA; 67 kDa laminin receptor; 67LR; colon carcinoma laminin-binding protein; ICAS; LAMBR; Laminin receptor 1; laminin receptor 1 (67kD, ribosomal protein SA); laminin-binding protein precursor p40; lamR; LAMR1; LBP; LBP/p40; LRP; LRP/LR; multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; p40; SA; small ribosomal subunit protein uS2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.