Rabbit anti-RSK1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-458A-T maps to a region between residue 375 and 425 of human 90 kDa ribosomal protein S6 kinase 1 using the numbering given in entry NP_002944.2 (GeneID 6195).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RSK1 immobilized on solid support.
The epitope recognized by A302-458A maps to a region between residue 375 and 425 of human 90 kDa ribosomal protein S6 kinase 1 using the numbering given in entry NP_002944.2 (GeneID 6195).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Ribosomal protein S6 kinase alpha-1 (RSK1) is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. RSK1 kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signaling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation [taken from NCBI Entrez Gene (GeneID: 6195)].
Alternate Names
90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); HU-1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK1A; MAPKAPK-1a; p90Rsk; p90-RSK 1; p90RSK1; p90S6K; ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal S6 kinase 1; RSK; RSK1; RSK-1; S6K-alpha 1; S6K-alpha-1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.