Rabbit anti-RUVBL1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-717A-T maps to a region between residue 402 to 452 of human RuvB-like AAA ATPase 1 using the numbering given in entry NP_003698.1 (GeneID 8607).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RUVBL1 immobilized on solid support.
The epitope recognized by A304-717A maps to a region between residue 402 to 452 of human RuvB-like AAA ATPase 1 using the numbering given in entry NP_003698.1 (GeneID 8607).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
RuvB-like 1 (RUVBL1) possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y265].
Alternate Names
49 kDa TATA box-binding protein-interacting protein; 49 kDa TBP-interacting protein; 54 kDa erythrocyte cytosolic protein; ECP54; ECP-54; epididymis secretory sperm binding protein; INO80 complex subunit H; INO80H; NMP 238; NMP238; nuclear matrix protein 238; PONTIN; pontin 52; Pontin52; RuvB (E coli homolog)-like 1; ruvB-like 1; RuvB-like AAA ATPase; RVB1; TAP54-alpha; TATA binding protein interacting protein 49 kDa; TIH1; TIP49; TIP49A; TIP60-associated protein 54-alpha
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.