Rabbit anti-RuvBL2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-537A-T maps to a region between residue 413 and 463 of human RuvB-like 2 using the numbering given in entry NP_006657.1 (GeneID 10856).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RuvBL2 immobilized on solid support.
The epitope recognized by A302-537A maps to a region between residue 413 and 463 of human RuvB-like 2 using the numbering given in entry NP_006657.1 (GeneID 10856).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
RuvB-like 2 (RuvBL2) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this protein has both ATPase and DNA helicase activities [taken from NCBI Entrez Gene (GeneID: 9400)].
Alternate Names
48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; CGI-46; ECP51; ECP-51; erythrocyte cytosolic protein, 51-KD; INO80 complex subunit J; INO80J; repressing pontin 52; REPTIN; Reptin 52; reptin52 protein; RuvB (E coli homolog)-like 2; ruvB-like 2; RVB2; TAP54-beta; TIH2; TIP48; TIP49B; TIP60-associated protein 54-beta
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.