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Rabbit anti-SAPS3 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
SAPS3
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 475 and 525
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to SAPS3 immobilized on solid support.

The epitope recognized by A300-971A maps to a region between residue 400 and 450 of human SAPS domain family, member 3 using the numbering given in entry NP_060782.2 (GeneID 55291).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to SAPS3 immobilized on solid support.

The epitope recognized by A300-971A-T maps to a region between residue 400 and 450 of human SAPS domain family, member 3 using the numbering given in entry NP_060782.2 (GeneID 55291).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

SAPS3 is a regulatory subunit of the PP6 phosphatase. There are 3 human SAPs (sit4-associated proteins), SAPS1, SAPS2, and SAPS3. The human SAPs are related to SAPs found in yeast that associate with the sit4 phosphatase which is the homolog of human PP6 phosphatase. In yeast sit4 and its associated SAPs are involved in a variety of processes which include transcription, translation, bud formation, glycogen metabolism, ion homeostasis, and telomere function. Because the SAPS3 gene maps to the IDDM4 locus at 11q13, SAPS3 has been implicated in insulin-dependent diabetes mellitus. SAPS domain family member 3, protein phosphatase 6 regulatory subunit 3, PP6R3, and KIAA1558.

55291
PPP6R3
protein phosphatase 6 regulatory subunit 3
Q5H9R7
Serine/threonine-protein phosphatase 6 regulatory subunit 3

Alternate Names

C11orf23; PP6R3; SAP190; SAPL; SAPLa; SAPS domain family member 3; SAPS domain family, member 3; SAPS3; serine/threonine-protein phosphatase 6 regulatory subunit 3; sporulation-induced transcript 4-associated protein SAPL

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.