Rabbit anti-SEC24B Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-877A maps to a region between residue 300 to 350 of human Protein transport protein Sec24B using the numbering given in entry O95487.2 (GeneID 10427).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SEC24B immobilized on solid support.
The epitope recognized by A304-877A-T maps to a region between residue 300 to 350 of human Protein transport protein Sec24B using the numbering given in entry O95487.2 (GeneID 10427).
Additional Product Information
SEC24B is a member of the SEC24 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. SEC24B has similarity to yeast Sec24p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The role SEC24B is implicated in the shaping of the vesicle, and also in cargo selection and concentration [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O95487].
Alternate Names
protein transport protein Sec24B; SEC24; SEC24 related gene family, member B; SEC24-related protein B; secretory protein 24
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.