Rabbit anti-SEC63 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-084A maps to a region between residue 700 to 750 of human Translocation protein SEC63 homolog using the numbering given in entry Q9UGP8.2 (GeneID 11231).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SEC63 immobilized on solid support.
The epitope recognized by A305-084A-T maps to a region between residue 700 to 750 of human Translocation protein SEC63 homolog using the numbering given in entry Q9UGP8.2 (GeneID 11231).
Additional Product Information
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Translocation protein SEC63 homolog, encoded by the gene SEC63, and the SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway [taken from NCBI Entrez Gene (Gene ID: 11231)].
Alternate Names
DNAJC23; ERdj2; PCLD2; PRO2507; SEC63 protein translocation regulator; SEC63L; SEC63-like protein; translocation protein SEC63 homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.