Rabbit anti-SF3A3 IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00710 maps to a region between residue 451 and 501 of human splicing factor 3a, subunit 3, 60kDa using the numbering given in entry NP_006793.1 (GeneID 10946).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SF3A3 immobilized on solid support.
The epitope recognized by IHC-00710-T maps to a region between residue 451 and 501 of human splicing factor 3a, subunit 3, 60kDa using the numbering given in entry NP_006793.1 (GeneID 10946).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
SF3A3 is one of 3 subunits of the SF3a splicing factor. SF3a is composed of a 60 kDa subunit (SF3A3/SF3a60), 66 kDa subunit (SF3A2/SF3a66), and a120 kDa subunit (SF3A1/SF3a120). SF3a is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing [taken from NCBI Entrez Gene (GeneID: 10946)].
Alternate Names
pre-mRNA splicing factor SF3a (60kD); PRP9; PRPF9; SAP 61; SAP61; SF3a60; spliceosome associated protein 61; Spliceosome-associated protein 61; splicing factor 3A subunit 3; splicing factor 3a, subunit 3, 60kD; splicing factor 3a, subunit 3, 60kDa
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.