Rabbit anti-SF3B3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-508A maps to a region between residue 1167 and 1217 of human splicing factor 3b, subunit 3 using the numbering given in entry NP_036558.3 (GeneID 23450).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SF3B3 immobilized on solid support.
The epitope recognized by A302-508A-T maps to a region between residue 1167 and 1217 of human splicing factor 3b, subunit 3 using the numbering given in entry NP_036558.3 (GeneID 23450).
Additional Product Information
SF3B3 is subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome [taken from NCBI Entrez Gene (GeneID: 23450)].
Alternate Names
pre-mRNA splicing factor SF3b, 130 kDa subunit; Pre-mRNA-splicing factor SF3b 130 kDa subunit; RSE1; SAP 130; SAP130; SF3b130; spliceosome-associated protein 130; splicing factor 3B subunit 3; STAF130
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.