Bethyl Laboratories® Rabbit anti-SHMT2 Antibody, Affinity Purified - 100 µl (1000 µg/ml)
Product Details
Specifications
Additional Product Information
Serine hydroxymethyltransferase (SHMT2) is the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. SHMT2 is primarily responsible for glycine synthesis. SHMT2 activity has been suggested to be the primary source of intracellular glycine [taken from NCBI Entrez Gene (Gene ID: 6472)].
Alternate Names
epididymis secretory sperm binding protein Li 51e; GLY A+; GLYA; glycine auxotroph A, human complement for hamster; glycine hydroxymethyltransferase; HEL-S-51e; serine aldolase; serine hydroxymethylase; serine hydroxymethyltransferase 2 (mitochondrial); serine hydroxymethyltransferase, mitochondrial; serine methylase; SHMT; threonine aldolase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.