Rabbit anti-SHMT2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-351A maps to a region between residue 454 to 504 of human Serine hydroxymethyltransferase, mitochondrial using the numbering given in entry P34897.3 (GeneID 6472).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SHMT2 immobilized on solid support.
The epitope recognized by A305-351A-T maps to a region between residue 454 to 504 of human Serine hydroxymethyltransferase, mitochondrial using the numbering given in entry P34897.3 (GeneID 6472).
Additional Product Information
Serine hydroxymethyltransferase (SHMT2) is the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. SHMT2 is primarily responsible for glycine synthesis. SHMT2 activity has been suggested to be the primary source of intracellular glycine [taken from NCBI Entrez Gene (Gene ID: 6472)].
Alternate Names
epididymis secretory sperm binding protein Li 51e; GLY A+; GLYA; glycine auxotroph A, human complement for hamster; glycine hydroxymethyltransferase; HEL-S-51e; serine aldolase; serine hydroxymethylase; serine hydroxymethyltransferase 2 (mitochondrial); serine hydroxymethyltransferase, mitochondrial; serine methylase; SHMT; threonine aldolase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.