Rabbit anti-Sin3A Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-725A-T maps to a region between residue 375 and 425 of human SIN3 homolog A using the numbering given in entry NP_056292.1 (GeneID 25942). Antibody was affinity purified using an epitope specific to Sin3A immobilized on solid support.
The epitope recognized by A300-725A maps to a region between residue 375 and 425 of human SIN3 homolog A using the numbering given in entry NP_056292.1 (GeneID 25942).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Sin3A is a part of a histone deacetylase co-repressor complex that functions to stabilize complex members and regulate gene expression through deacetylation of histones. Sin3A acts as a scaffold for a core complex which contains HDAC1, HDAC2, RbAp46, RbAp48, SAP30, RBP1 and p33INHib. Sin3A also associates with the Mad/Max complex to mediate transcriptional repression.
Alternate Names
histone deacetylase complex subunit Sin3a; paired amphipathic helix protein Sin3a; SIN3 homolog A, transcription regulator; transcriptional corepressor Sin3a; transcriptional co-repressor Sin3A; transcriptional regulator, SIN3A; WITKOS
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.