Rabbit anti-SMARCD1/BAF60a Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-595A-T maps to a region between residue 250 and 300 of human , SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 using the numbering given in entry NP_003067.2 (GeneID 6602). Antibody was affinity purified using an epitope specific to SMARCD1/BAF60a immobilized on solid support.
The epitope recognized by A301-594A maps to a region between residue 250 and 300 of human , SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 using the numbering given in entry NP_003067.2 (GeneID 6602).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. SMARCD1/BAF60a is subunit of the SWI/SNF complex that has been identified as a factor that interacts with nuclear receptors such as the vitamin D receptor and glucocorticoid receptor and a subset of Fos/Jun dimers. SMARCD1 has also been shown to interact with p53 and is suggested to be involved in the tumor suppressor activity of p53. From these findings it has been concluded that SMARCD1 functions as a direct link between transcription factors and SWI/SNF remodeling activity to regulate transcription.
Alternate Names
60 kDa BRG-1/Brm-associated factor subunit A; BAF60A; BRG1-associated factor 60A; chromatin remodeling complex BAF60A subunit; CRACD1; mammalian chromatin remodeling complex BRG1-associated factor 60A; Rsc6p; SWI/SNF complex 60 kDa subunit; SWI/SNF complex 60 kDa subunit A; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1; Swp73-like protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).