Rabbit anti-SMC1 Recombinant Monoclonal Antibody [BL-205-2G8]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Immunogen was a synthetic peptide representing a region between residue 1200 and the C-terminus (residue 1233) of human structural maintenance of chromosomes 1 according to the numbering given in entry NP_006297.2 (GeneID 8243). Recombinant antibody was purified from cell culture supernatant.
Immunogen was a synthetic peptide representing a region between residue 1200 and the C-terminus (residue 1233) of human structural maintenance of chromosomes 1 according to the numbering given in entry NP_006297.2 (GeneID 8243).
Additional Product Information
Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a heterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holds sister chromatids together. Cohesin is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination.
Alternate Names
CDLS2; DXS423E; epididymis secretory sperm binding protein; SB1.8; segregation of mitotic chromosomes 1; SMC protein 1A; SMC1; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC-1A; SMC1alpha; SMC-1-alpha; SMC1L1; SMCB; structural maintenance of chromosomes protein 1A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.