Rabbit anti-SMC3 Recombinant Monoclonal Antibody [BLR099G]
Product Details
Specifications
Immunogen was a peptide representing a region between residue 1175 and the C-terminus (residue 1217) of human Structural maintenance of chromosomes protein 3 (SMC3) using the numbering given in entry NP_005436.1 (Gene ID 9126).
Additional Product Information
SMC3 (structural maintenance of chromosomes 3) is a component of the cohesin complex which is critical for precise duplication and segregation of chromosomes. SMC3 heterodimerizes with SMC1 and associates with two non-SMC subunits, Rad21/Scc1 and Scc3, to form the cohesin complex.
Alternate Names
BAM; Bamacan; basement membrane-associated chondroitin proteoglycan; BMH; CDLS3; Chondroitin sulfate proteoglycan 6; chondroitin sulfate proteoglycan 6 (bamacan); chromosome-associated polypeptide; CSPG6; HCAP; SMC protein 3; SMC-3; SMC3L1; structural maintenance of chromosomes protein 3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.