Rabbit anti-SMC6 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-237A-M maps to a region between residues 1050 and the C-terminus (residue 1091) of human Structural Maintenance of Chromosomes 6 using the numbering given in TrEMBL entry Q96SB8 (GeneID 79677).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
SMC (Structural maintenance of chromosomes) proteins are members of multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. Two of these complexes, cohesin and condensin consist of a core complex of an SMC1 and SMC3 heterodimer or an SMC2 and SMC4 heterodimer, respectively. A third complex includes a heterodimer of SMC5 and SMC6 whose function is not fully understood but is proposed to be related to DNA repair.
Alternate Names
hSMC6; SMC protein 6; SMC-6; SMC6 structural maintenance of chromosomes 6-like 1; SMC6L1; structural maintenance of chromosomes protein 6
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.