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Rabbit anti-SOX10 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
SOX10
Reactivity:
Human, Mouse
Platforms:
PhenoImager™ HT
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 416 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Chicken, Pig, X. laevis, X. tropicalis, Rat
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to SOX10 immobilized on solid support. The epitope recognized by A305-799A maps to a region between residue 416 and 466 of human DOM; WS4; PCWH; WS2E; WS4C using the numbering given in entry P56693.1 (GeneID 6663).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to SOX10 immobilized on solid support.

The epitope recognized by A305-799A-T maps to a region between residue 416 and 466 of human DOM; WS4; PCWH; WS2E; WS4C using the numbering given in entry P56693.1 (GeneID 6663).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. SOX10 may act as a transcriptional activator after forming a protein complex with other proteins. SOX10 acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development [taken from NCBI Entrez Gene (Gene ID: 6663)].

6663
SOX10
SRY-box 10
P56693
Transcription factor SOX-10

Alternate Names

DOM; dominant megacolon, mouse, human homolog of; PCWH; SRY (sex determining region Y)-box 10; SRY-related HMG-box gene 10; transcription factor SOX-10; WS2E; WS4; WS4C

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.