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Rabbit anti-SPC24 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
SPC24
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to SPC24 immobilized on solid support.

The epitope recognized by A304-260A maps to a region between residue 1 to 50 of human Spindle Pole Body Component 24 using the numbering given in entry NP_872319.1 (GeneID 147841).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to SPC24 immobilized on solid support.

The epitope recognized by A304-260A-T maps to a region between residue 1 to 50 of human Spindle Pole Body Component 24 using the numbering given in entry NP_872319.1 (GeneID 147841).
Country of Origin
USA

Additional Product Information

Kinetechore protein SPC24 acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. SPC24 is required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8NBT2].

147841
SPC24
SPC24, NDC80 kinetochore complex component
Q8NBT2
Kinetochore protein Spc24

Alternate Names

hSpc24; kinetochore protein Spc24; SPBC24; SPC24, NDC80 kinetochore complex component, homolog; spindle pole body component 24 homolog

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).