Rabbit anti-SPHK1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-177A maps to a region between residue 334 and 384 of human sphingosine kinase 1 using the numbering given in entry NP_001136073.1 (GeneID 8877).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SPHK1 immobilized on solid support.
The epitope recognized by A302-177A-T maps to a region between residue 334 and 384 of human sphingosine kinase 1 using the numbering given in entry NP_001136073.1 (GeneID 8877).
Additional Product Information
Sphingosine kinase 1 (SPHK1) catalyzes the phosphorylation of sphingosine to yield sphingosine-1-phosphate (SPP). SPP functions as a lipid signaling molecule that mediates both intra- and extra-cellular responses. SPHK1activity may play a role in several cellular processes such as apoptisis, proliferation, inflammation, and migration.
Alternate Names
SK 1; sphingosine kinase 1; SPHK; SPK 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.