Rabbit anti-SPTLC1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog # A303-407A
Validated
Target:
SPTLC1
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 50 and 100
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
Detection of human and mouse SPTLC1 by western blot (h and m) and immunoprecipitation (h).
1 Figure
100 µl (200 µg/ml)

10 µl (200 µg/ml)

A303-407A
$464.00
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Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Bovine
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to SPTLC1 immobilized on solid support.

The epitope recognized by A303-407A maps to a region between residue 50 and 100 of human Serine Palmitoyltransferase, Long Chain Base Subunit 1 using the numbering given in entry NP_006406.1 (GeneID 10558).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Serine palmitoyltransferase (SPT), which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPTLC1 (serine palmitoyltransferase, long chain base subunit 1 is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in SPTLC1 were identified in patients with hereditary sensory neuropathy type 1 [taken from NCBI Entrez Gene (Gene ID: 10558)].

10558
SPTLC1
serine palmitoyltransferase long chain base subunit 1
O15269
Serine palmitoyltransferase 1

Alternate Names

HSAN1; HSN1; LBC1; LCB 1; LCB1; long chain base biosynthesis protein 1; serine C-palmitoyltransferase; serine palmitoyltransferase 1; serine-palmitoyl-CoA transferase 1; SPT 1; SPT1; SPTI

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunoprecipitation (IP)
6 µg/mg lysate
Western Blot (WB)
1:2000 - 1:10,000

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