Rabbit anti-SRF Recombinant Monoclonal Antibody [BLR308M]
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 25 and 75 of human Serum response factor using the numbering given in entry NP_003122.1 (Gene ID 6722).
Additional Product Information
Serum response factor (SRF) is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). SRF is required for cardiac differentiation and maturation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P11831].
Alternate Names
c-fos serum response element-binding transcription factor; MCM1; minichromosome maintenance 1 homolog; serum response factor; SRF
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.