Rabbit anti-STAT1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-752A maps to a region between residue 175 and 225 of human Signal Transducer and Activator of Transcription 1 using the numbering given in entry NP_009330.1 (GeneID 6772).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to STAT1 immobilized on solid support.
The epitope recognized by A302-752A-T maps to a region between residue 175 and 225 of human Signal Transducer and Activator of Transcription 1 using the numbering given in entry NP_009330.1 (GeneID 6772).
Additional Product Information
STAT1 (signal transducer and activator of transcription 1) is a member of the STAT protein family). In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens [taken from NCBI Entrez Gene (GeneID: 6772)].
Alternate Names
CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription 1-alpha/beta; STAT91; transcription factor ISGF-3 components p91/p84
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.