Rabbit anti-STAT5a Recombinant Monoclonal Antibody [BLR124H]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 744 and the C-terminus (residue 794) of human signal transducer and activator of transcription 5a using the numbering given in entry NP_001275647.1 (Gene ID 6776).
Additional Product Information
Signal transducer and activator of transcription 5a (STAT5a) is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones [taken from NCBI Entrez Gene (Gene ID: 6776)].
Alternate Names
epididymis secretory sperm binding protein; MGF; signal transducer and activator of transcription 5A; STAT5
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.