Rabbit anti-Tat-SF1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-023A maps to a region between residue 375 and 425 of human HIV-1 Tat specific factor 1 using the numbering given in entry NP_055315.2 (GeneID 27336).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Tat-SF1 immobilized on solid support.
The epitope recognized by A302-023A-T maps to a region between residue 375 and 425 of human HIV-1 Tat specific factor 1 using the numbering given in entry NP_055315.2 (GeneID 27336).
Additional Product Information
Tat-SF1 was originally identified as a cellular factor required for the trans-activation of HIV-transcription by the HIV protein, Tat. In the cell, Tat-SF1 functions as a general transcription factor that plays a role in transcriptional elongation.
Alternate Names
cofactor required for Tat activation of HIV-1 transcription; dJ196E23.2; HIV TAT specific factor 1; HIV Tat-specific factor 1; TATSF1; TAT-SF1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.