Rabbit anti-TBCA Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-553A maps to a region between residue 58 to 108 of human Tubulin-specific chaperone A using the numbering given in entry O75347.3 (GeneID 6902).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TBCA immobilized on solid support.
The epitope recognized by A305-553A-T maps to a region between residue 58 to 108 of human Tubulin-specific chaperone A using the numbering given in entry O75347.3 (GeneID 6902).
Additional Product Information
Tubulin-specific chaperone A (TBCA) is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state [taken from NCBI Entrez Gene (Gene ID: 6902)].
Alternate Names
CFA; chaperonin cofactor A; epididymis secretory sperm binding protein; TCP1-chaperonin cofactor A; Tubulin-folding cofactor A; tubulin-specific chaperone A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.