Rabbit anti-TCP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-443A-T maps to a region between residue 150 and 200 of human T-Complex 1 using the numbering given in entry NP_110379.2 (GeneID 6950).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TCP1 immobilized on solid support.
The epitope recognized by A303-443A maps to a region between residue 150 and 200 of human T-Complex 1 using the numbering given in entry NP_110379.2 (GeneID 6950).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
T-complex protein 1 subunit alpha (TCP1-alpha) is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin [taken from NCBI Entrez Gene (Gene ID: 6950)].
Alternate Names
CCT1; CCTa; CCT-alpha; D6S230E; tailless complex polypeptide 1; t-complex 1 protein; T-complex protein 1 subunit alpha; T-complex protein 1, alpha subunit; TCP-1-alpha
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.